Resumo

AFIEROHO, Ozadheoghene E et al. An antiplasmodial depside from a nigerian lichen dirinaria picta, epiphytic on the oil palm Elaeis Guineense. Rev. Bol. Quim [online]. 2018, vol.35, n.1, pp.31-39. ISSN 0250-5460.

This study investigated the anti-plasmodial and cytotoxic potentials of the chloroform (LCE) and ethanol (LEE) extracts from the foliose lichen Dirinaria picta with the view of isolating anti-malarial drug lead compound(s). In vitro anti-plasmodial and cytotoxicity assays were done using the plasmodium lactate dehydrogenase assay and human HeLa cervica cell lines respectively. The structure of the isolated compound was elucidated using spectroscopic techniques. The LCE yielded a novel antiplasmodial depside 1 (antiplasmodial IC₅₀≈37 (μg/mL; cytotoxicity (IC₅₀ >100 μg/mL; Selectivity index >2.7) and an impure fraction LC2 (antiplasmodial IC₅₀ ≈ 79 μg/mL; cytotoxicity (IC₅₀ >100 μg/mL; Selectivity index <1.3). The LEE (antiplasmodial IC₅₀ ≈ 17 μg/mL; cytotoxicity (IC₅₀ ≈ 62 μg/mL; Selectivity index ≈ 3.7) showed a significantly (p < 0.05) better anti-plasmodial activity though more cytotoxic compared to depside 1 and LC2.The depside 1, LC2 and LEE were less cytotoxic compared to emetine (cytotoxicity (IC₅₀ = 0.02 μM ≈ 0.013 μg/mL) though not as active as the reference drugs chloroquine (antiplasmodial IC₅₀ = 0.031 μM ≈ 0.016 μg/mL). This is the first time report on the anti-malarial potential of Nigerian lichens and the isolation of a novel anti-plasmodial depside 1.

Palavras-chave : Lichens; Depside; Anti-plasmodial; Cytotoxicity; Drug discovery.

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