Resumo

ROCABADO CALIZAYA, Guillermo; DE LA FUENTE VASQUEZ, Francisco  e  NIETO MARTINEZ, Juan Patricio. DETERMINATION BY LC-MS/MS ETILGLUCORONIDO, POSSIBLE INDICATOR IN FORENSIC ANALYSIS. Rev. Méd. La Paz [online]. 2012, vol.18, n.1, pp.5-8. ISSN 1726-8958.

D-etilglucuronido (EtG) stable metabolite of ethanol, can be identified in urine up to 3.5 days after ingestion and may be useful for the diagnosis of intake in forensic studies, were used for determination LC-MS/MS systems. The target urine tests and those treated with EtG and samples and following the analytical methodology described in the study, we observed the presence of ions, the most abundant being those derived from D5-EtG and EtG (m/z 75, 85, and 113) are the result of glucuronic acid aglycone not including ethanol. As can be noted that these ions with low abundance are the result of lactonizatión or water loss (m/z 203), additional decarboxylation (m/z 159), loss of ethanol (m/z 113), and water or CO. For all the proven, EtG able to identify and use it as an indicator of the presence of ethanol in the body, to extrapolate data from consumption of ethanol in forensic studies suggest that this methodology could be used to clarify the conflicts this substance.

Palavras-chave : EtG Etilglucurónido; LC Cromatografía liquida; MS espectrometría de masas.

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